Blue pippin. Although they're called halflings, hobbits are a little more...

Gels were run using the Pippin Pulse pulsed-˚eld electrophoresis

selected protocol will be highlighted in blue. g. For the target duration, enter the desired run time (typically 14 to 16 hours). Note that the default value is 2 hours. Consider optimizing the run time based on the ambient lab temperature since the Pippin Pulse Electrophoresis System does not regulate the temperature in the gel chamber.selected protocol will be highlighted in blue. g. For the target duration, enter the desired run time (typically 14 to 16 hours). Note that the default value is 2 hours. Consider optimizing the run time based on the ambient lab temperature since the Pippin Pulse Electrophoresis System does not regulate the temperature in the gel chamber.Meriadoc Brandybuck and Peregrin Took (better known as Merry and Pippin) are incredibly close friends, not to mention that they are also first cousins — Esmeralda Brandybuck, Merry's mother, happens to be Paladin Took's younger sister. Together, they make the naughtiest, yet most adorable, pair in all of Hobbiton, probably even the Shire.The Bioinformatics Core is offering in-depth analyses of PacBio sequence data. Samples can be provided as already isolated DNA by the customers. We do offer HMW-DNA isolation services for PacBio sequencing. Real-time data collection uses a 4 hour movie capture of the SMRT cell. Movies are processed in real time into trace files for each ZMW ...It's important to carefully inspect gel cassettes prior to use on the Pippin Prep or BluePippin. This instructional video from Sage Science shows how to quic...BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.Blue Pippin Standard DNA size selection cassette 2% agarose The blue pippin is a machine that is used to size-select DNA fragments from 100bp-50kbp.Target sizes or rangesof sizes are entered in software, and fractions are collected in buffer.For each Nanopore library, the gDNA was size-selected (10-50 kb) with a Blue Pippin system (Sage Science, USA) and processed using the Ligation Sequencing 1D kit (SQKLSK109, Oxford Nanopore Technologies, UK) according to the manufacturer's instructions. Library construction and sequencing were done by the GridION X5/PromethION sequencer ...Sketch about a Blue Peter spoof talking about Jason, and his unfortunate circumstances. Subscribe for more: http://bit.ly/hattricksubThe show collected stamp...Buckley lab protocols for conducting SIP studies. Contribute to nick-youngblut/Buckley_Lab_SIP_project_protocols development by creating an account on GitHub.Background Bacillus cereus is ubiquitous in nature, found in environments such as soil, plants, air, and part of the insect and human gut microbiome. The ability to produce endospores and biofilms contribute to their pathogenicity, classified in two types of food poisoning: diarrheal and emetic syndromes. Here we report gap-free, whole …BluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is an50 years ago, on October 23, 1972, the Broadway cast of a new musical called Pippin had parts to perform and hearts to warm on their opening night at the Imperial Theatre. For the show's composer and lyricist, Stephen Schwartz, fresh off the success of Godspell Off-Broadway, the opening marked the premiere of his first Broadway musical. Pippin follows the journey of a young prince searching ...2.0 BLUE-PIPPIN INSTALLATION REQUIREMENTS 2.1 Operating Conditions The BluePippin system is designed to operate under the following environmental conditions: Pollution Degree 2 Installation category 2 Max. Altitude 2000m Indoor use Ambient temperature 17-25oC Humidity 10-80%, non-condensing 2.2 SafetyPipkin's Market, Montgomery, Ohio. 4,794 likes · 10 talking about this · 882 were here. Your neighborhood Farmer's Market, only better! Fresh Produce & Curated Garden CenterDefine pippin. pippin synonyms, pippin pronunciation, pippin translation, English dictionary definition of pippin. n. 1. Any of several varieties of apple. 2. The seed of a fleshy fruit; a pip. 3. Informal A person or thing that is admired. American Heritage® Dictionary...Are you looking to get the most out of your 2007 Honda Civic? The Blue Book is a great resource to help you do just that. The Blue Book is an online resource that provides information on the value of used cars, including the 2007 Honda Civi...Repaired DNAs were selected by a Blue Pippin system to construct SMRTbell DNA libraries with fragment sizes of >10 kb according to the manufacturer’s specification (PacBio, Menlo Park, CA, United States). Library quality was determined using a Qubit 2.0 Fluorometer (Life Technologies, CA, United States), and the average fragment size ...Library DNA was size-selected using a 2% agarose gel cassette of Blue Pippin (Sage Science, Beverley, MA, USA) for a target size range 150-300 bp and quantified with droplet digital PCR (QX-200; BioRad, Hercules, CA, USA). Sequencing was performed on an Illumina HiSeq 4000 to generate 150-nucleotide, single-end reads at a read depth of at least ...Size selection with Blue-Pippin system (Sage Science) to remove fragments less than 15 Kb was done on purified sample with 0.45X AMPure PB beads (Pacific Biosciences). The size and concentration ...For PacBio libraries, we followed the study of Chakraborty et al. by shearing the DNA using a 1.5-inch 24-gauge blunt-tipped needle, and the library was prepared using PacBio's SMRTbell template protocol and Blue Pippin size selection using a 15- to 50-kb cutoff. We generated several dozen libraries in this manner and collected 124 total ...Background Bacillus cereus is ubiquitous in nature, found in environments such as soil, plants, air, and part of the insect and human gut microbiome. The ability to produce endospores and biofilms contribute to their pathogenicity, classified in two types of food poisoning: diarrheal and emetic syndromes. Here we report gap-free, whole …The BluePippin is a DNA size selection system from Sage Science. DNA size selection for next generation sequencing allows for separation from 100 bp – 50 kb using a pulsed-field …Sage Science bluepippin device Bluepippin Device, supplied by Sage Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and moreThis instructional video from Sage Science guides you through the process of preparing your cassette for use with either the Pippin Prep or BluePippin automa...Find the nearest stores. Search Address. Use current location. Seiko Prospex remains a standard for high-intensity timekeeping excellence. From the introduction of its first diver's watch in 1965, Seiko has been a leader in undersea precision and durability, spearheading innovations in both technology and design.The PippinHT system is the top choice for higher-throughput workflows. The PippinHT can process 11 size selections at once on a gel cassette (1 of the 12 lanes must be dedicated to an external size marker). The PippinHT accommodates 2 gel cassettes so up to 22 samples can be run at once, and at a quarter of the run time (1 hr 15 m) when ...library was size-selected using a Blue Pippin (Sage Science), retaining only those fragments 300 bp ± 50 bp in length. This size-selection range was chosen by performing an in silico diges-tion of the published prairie rattlesnake, C. viridis viridis, genome [43], with the cut-site sequences of PstI (CTGCA^G) and MspI (C^CGG) using the program ...Enjoy a lovely breakfast with this blue Blushing Birds breakfast set which contains 3 items. Within this set you will find a breakfast plate, ...98 subscribers Subscribe 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the...blue light for visualization. Do not use ethidium bromide and/or UV light since they can induce DNA damage. The SYBR stains can be easily removed from nucleic acids during the gel-extraction process, e.g., using Qiagen® gel-extraction kits. 2) Purity of your DNA sample: DNA purity can be determined by using the NanoDrop® instrument or otherThe Blue Pippin is used to select DNA of certain sizes and effectively remove unwanted fragment sizes. If you intend to use Illumina technology, we will select fragments in a target size range …All libraries were constructed at BGI (Shenzhen, China). For PacBio sequencing, genomic DNA from the Tuantuan was used to construct three libraries of 20-kb insert size using the SMRTbell Template Prep Kit 1.0 (Pacific Biosciences, USA), and the fragment size was selected using Blue Pippin (Labgene Scientific SA, Switzerland).Blue pippin size selection elution volume 07-03-2015, 02:20 AM. Hello, Has anyone has tried eluting their TruSeq DNA libraries in a smaller volume than the 40uL stated in the protocol? We carry out size selection on our TruSeq nano libraries after the PCR enrichment step which works for us but gives very low concentrations. I was wondering if ...6.4.2 Iso-Seq, a Tool to Use to Survey for New Isoforms. Dr. Snyder's group demonstrated that the Iso-Seq technology is capable of sequencing full-length RNA, with little to no sequence loss at the 5′ end. In their paper, the authors were able to sequence transcripts without fragmentation or amplification.The Blue-headed Vireo is a small bird with a body length of 12.6-14.8 cm as well as a wing of 20.0-24.0 cm. An adolescent Blue-headed Vireo measures about 13 and 19g on normal. The Blue-headed Vireo has an estimated lifetime of seven years plus five months. The Blue-headed Vireo is an insectivorous bird, which means it eats large insects.After this, PCR reamplification of the captured DNA pool with spacer-acquisition-specific primers allows visible detection of spacer acquisition in just 1 in 10 5 cells (0.001%) in the population ...About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features NFL Sunday Ticket Press Copyright ...Check out our blue pippin fabric selection for the very best in unique or custom, handmade pieces from our fabric shops.Libraries were prepared with Illumina TruSeq Stranded mRNA kit starting from 600 ng of total RNA, and samples were fragmented and amplified for 15 PCR cycles. Libraries were size selected with Blue Pippin (Sage Science) using 1.5% gel cassettes and 350–550 bp regions isolated.The BluePippin system uses dye-free, pre-cast gel cassettes to separate and extract desired DNA fragment size ranges from agarose. It enables size selection for fragment lengths between 90 bp to 50 kb using different gel cassettes. The BluePippin system can run pulsed-field gel electrophoresis programs and thus separate large DNA fragments.Finally, large fragments (>10 kb) were enriched with Blue Pippin device (Sage Science, Inc., Beverly, MA, USA) for sequencing. A total of 15 single-molecule real-time (SMRT) cells were processed, 7 with Sequel and 8 with RS II instruments (PacBio), to generate a total of 67.32 Gb PacBio data. For Oxford Nanopore sequencing, ∼5 μg of genomic ...The Apple Pippin is a defunct open multimedia technology platform, designed by Apple Computer, and marketed as PiPP!N. According to Apple, Pippin was directed at the home market as "an integral part of the consumer audiovisual, stereo, and television environment." ... Sonoran Blue 1996 NA, JP WebViewer with MS Internet Explorer: Non-game, Web ...3.0 BLUE-PIPPIN INSTALLATION PROCEDURE 3.1 Packaging and Components The system is shipped in two boxes: one will contain the BluePippin instrument and accessories, and the second box will contain the computer monitor in the manufacturer's original packaging. With the boxes in the upright position, open and confirm that the following items are ...Description. The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long …The Bioinformatics Core is offering in-depth analyses of PacBio sequence data. Samples can be provided as already isolated DNA by the customers. We do offer HMW-DNA isolation services for PacBio sequencing. Real-time data collection uses a 4 hour movie capture of the SMRT cell. Movies are processed in real time into trace files for each ZMW ...Blue Pippin size selection, high MW DNA selection : $36 per sample: $59 per sample: DNA/RNA quantification. More info about DNA/RNA quantification. DNA/RNA quantification; Description Internal price (Cornell and Cornell affiliates) External price; Digital PCR, Illumina library quantification, reactions set up by the Genomics Facility:BluePippin and Pippin Prep Rinse Cassette. Sage Science SKU: SAG-720040. Price: $55.Size-selection was performed using the Blue Pippin electrophoresis platform (Sage Science), in order to select fragments of 215 bp of length as in [ 70 ], in order to be able to integrate our data ...Page 2 P/N 100-286-000-07 STEP Concentrate DNA Notes 1 Add 0.45X volume of AMPure® PB magnetic beads. _____ μL of sample X 0.45X = _____ μL of beads Note that the beads must be brought to room temperature and all AMPure PB beadIntroductionSugarcane is one of the most important sugar crops worldwide, however, sugarcane production is seriously limited by sugarcane red rot, a soil-borne disease caused by Colletotrichum falcatum. Bacillus velezensis YC89 was isolated from sugarcane leaves and can significantly inhibited red rot disease caused by C. falcatum.MethodsIn this study, the genome of YC89 strain was sequenced ...The Blue-headed Vireo is a small bird with a body length of 12.6-14.8 cm as well as a wing of 20.0-24.0 cm. An adolescent Blue-headed Vireo measures about 13 and 19g on normal. The Blue-headed Vireo has an estimated lifetime of seven years plus five months. The Blue-headed Vireo is an insectivorous bird, which means it eats large insects.The complementary DNA (cDNA) was synthesized using the Clontech SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA). Fragments >4 kb were selected using the Blue Pippin Size Selection System protocol (Sage Science, Beverly, MA, USA). The >4 kb cDNA and normal cDNAs were mixed in equimolar amounts for SMRT sequencing.PippinHT Quick Guide HTC2010 Marker 20B. 1MB. PippinHT Quick Guide HTC1510 Marker 15C. 1MB. PippinHT Quick Guide HPE7510 Marker 75E 6-10Kb and 15-20kb High Pass. 948KB. PippinHT Quick Guide HPF7510 Marker 75F >30-40kb High Pass. 1MB. PippinHT Quick Guide HRT7510 9-30kb Range +T Marker 75E.Size selection was performed at the University of Maryland Genomics Resource Center using a Blue Pippin pulse-field gel electrophoresis instrument. A library was constructed and 24 SMRT cells were sequenced on their PacBio RS II using the P5-C3 chemistry. ... The bottom (blue) track shows the testis transcriptome assembly aligned to M_zebra ...Blue Cross Bromsgrove rehoming centre, Bromsgrove. 11,320 likes · 141 talking about this · 986 were here. The Bromsgrove centre opened in 1986 and is now one of our largest centres. Located in...In most cases PCR bubble artifacts can not be removed by SPRI bead size selections or Blue Pippin size selections; if necessary, a "Reconditioning PCR" is the best option. However, to avoid unnecessary complexity loss of the library and introduction of polymerase errors, it would be best to optimize the library preparation protocol for a ...Abstract. The PacBio ® HiFi sequencing method yields highly accurate long-read sequencing datasets with read lengths averaging 10-25 kb and accuracies greater than 99.5%. These accurate long ...Product Type. Agarose Gel Cassette. Separation Range. 100 to 600 bp. Quantity. 10 Pack. Provide Content Correction. For size selections between 100bp and 600bp. Sage Science™ BluePippin™ 2% Agarose Gel Cassettes are commonly used for Illumina paired-end library construction, Ion Torrent template prep, and ChIP applications. "PIPPIN: I didn't think it would end this way. GANDALF: End? No, the journey doesn't end here. Death is just another path, one that we all must take. The grey rain-curtain of this world rolls back, and all turns to silver glass, and then you see it. PIPPIN: What? Gandalf? See what?The BluePippin is an updated version of Sage’s Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal ... ID Screen® Bluetongue Competition. Competitive ELISA kit for the detection of anti-VP7 antibodies in serum or plasma from multiple species. BTC-5P / BTC-10P. ID Screen® Bluetongue Milk Indirect. Indirect ELISA for the detection of anti-VP7 antibodies in individual or bulk milk samples. BTSMILK-4P.The ColourTex® stainless steel range is unique whereby the colours and tones of the product varies in different lighting conditions. This is known as a ‘living’ colour when viewed from different angles, also known as metamerism. The ColourTex® process can be combined with the surface finishes of mirror, satin and other polishes, Granex .... Figure Legend Snippet: Fragment analysis of cDNA (after libselected protocol will be highlighted in blue. g. For the target durat The haplotypes are colored by their broad grouping: (yellow) DR4, (blue) DR3, (purple) DR1, (red) DR2. White indicates gaps relative to the longest sequence alignment. Black shading indicates locations where the respective repeat element was identified. ID Screen® Bluetongue Competition. Comp The blue pippin is a machine that is used to size-select DNA fragments from 100bp-50kbp.Target sizes or rangesof sizes are entered in software, and fractions are collected in buffer. Inquire Blue Pippin Standard DNA size selection cassette 3% agarose The authors report on a high quality genome assembly of the model plant species Arabidopsis include two telomere-to-telomere assemblies (Chr 3 and 5). PacBio's HiFi, Oxford Nanopore ultra-long reads and Hi-C (Illumina) were used. BluePippin was used to size select large fragments from genomic extracts and for Hi-FI library construction. Blue Cross Blue Shield; Anthem; Passport; Avēsis; Hu...

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